Establishment of fingerprints and determination of various ingredients of yanlishuang pills by GC-MS

Yanlishuang Pills is a kind of traditional Chinese medicine used to treat pharyngitis widely. In this study, we gas chromatography tandem mass spectrometry (GC-MS) establish method for the fingerprint and quantitative analysis four major components Pills, which can provide more reliable its quality control. We software “Chromatographic Fingerprint Similarity Evaluation System Traditional Medicine”, version A, 2004, obtain using averaging with time width 0.1. The peak largest area was as reference determine shared peaks generate common pattern. Then main were identified their contents determined in GC-MS SIM mode internal standard method.The established by reproducible, total 18 13 batches samples, similarity each batch samples above 0.99. concentrations camphor, menthone, borneol menthol ingredients linearly well within range 25.13-150.78 μg/mL (r = 0.9995), 28.77-172.62 0.9991), 299.70-1798.20 0.9997), 121.98-731.88 average recoveries 102.02% (RSD 1.3%), 96.10% 1.0%), 102.71% 102.58% 1.1%), respectively, good precision, reproducibility, stability 16 h. camphor content 5.6025-8.3662 mg/g, menthone 4.7871-5.8936 88.0034-133.0969 mg/g 40.2017-61.9466 mg/g. fingerprints characterized pattern, simultaneous determination rapid, simple accurate. conclusion, multiple combined fingerprinting comprehensive control Pills.